Zebrafish Neurobehavioral Protocols

As a model organism, zebrafish (Danio rerio) offer an opportunity to perform steadfast scientific investigations in a robust and high-throughput manner. In Zebrafish Neurobehavioral Protocols, experts in the field provide a series of detailed behavioral protocols dealing with the major current research areas in the field of behavioral neuroscience. The chapters discuss the use and interpretation of video-aided quantification of zebrafish behaviors, describe novel assays commonly used to quantify emotionality, learning, memory, and social behaviors in zebrafish, and instruct how to quantify endocrine responses and correlate them with behavior. Written in the Neuromethods series format, the chapters provide authoritative reviews of many commonly used approaches in the field today. This "how to" book will be a useful practical guide for zebrafish researchers and will complement another related book from this series, Zebrafish Models in Neurobehavioral Research. Cutting-edge and timely, Zebrafish Neurobehavioral Protocols serves as an excellent compilation epitomizing the growing potential of zebrafish in modern scientific inquiry.

Written in the Neuromethods format, this book reviews protocols in behavioral neuroscience, including assays used to quantify emotionality, learning, memory, and social behaviors, and shows how to quantify endocrine responses and correlate them with behavior.

1. Video-Aided Analysis of Zebrafish Locomotion and Anxiety-Related Behavioral Responses Jonathan M. Cachat, Peter R. Canavello, Salem I. Elkhayat, Brett K. Bartels, Peter C. Hart, Marco F. Elegante, Esther C. Beeson, Autumn L. Laffoon, Whitlee A.M. Haymore, David H. Tien, Anna K. Tien, Sopan Mohnot, and Allan V. Kalueff 2. Videograms: A Method for Repeatable Unbiased Quantitative Behavioral Analysis Without Scoring or Tracking Russell C. Wyeth, Oliver R. Braubach, Alan Fine, and Roger P. Croll 3. Automated Imaging of Avoidance Behavior in Larval Zebrafish Ruth M. Colwill and Robbert Creton 4. Quantifying Anti-Predator Responses to Chemical Alarm Cues Brian D. Wisenden 5. Modified Associative Learning T-Maze Test for Zebrafish (Danio rerio) and Other Small Teleost Fish Georgianna G. Gould 6. Zebrafish Conditioned Place Preference Models of Drug Reinforcement and Relapse to Drug Seeking Amit Parmar, Miral Parmar, and Caroline H. Brennan 7. A Simple and Effective Method to Condition Olfactory Behaviors in Groups of Zebrafish Oliver R. Braubach, Russell C. Wyeth, Andrew Murray, Alan Fine, and Roger P. Croll 8. Aquatic Light/Dark Plus Maze Novel Environment for Assessing Anxious Vs. Exploratory Behavior in Zebrafish (Danio rerio) and Other Small Teleost Fish Georgianna G. Gould 9. A Novel Test Battery to Assess Drug-Induced Changes in Zebrafish Social Behavior David J. Echevarria, Christine Buske, Christina N. Toms, and David J. Jouandot 10. Measuring Agonistic Behavior in Zebrafish Henning Schneider 11. Measuring Endocrine (Cortisol) Responses of Zebrafish to Stress Peter R. Canavello, Jonathan M. Cachat, Esther C. Beeson, Autumn L. Laffoon, Chelsea Grimes, Whitlee A.M. Haymore, Marco F. Elegante, Brett K. Bartels, Peter C. Hart, Salem I. Elkhayat, David H. Tien, Sopan Mohnot, Hakima Amri, and Allan V. Kalueff 12. Phenotyping of Zebrafish Homebase Behaviors in Novelty-Based Tests Adam Stewart, Jonathan M. Cachat, Keith Wong, Nadine Wu, Leah Grossman, Christopher Suciu, Jason Goodspeed, Marco F. Elegante, Brett K. Bartels, Salem I. Elkhayat, David H. Tien, Siddharth Gaikwad, Ferdous Kadri, Kyung Min Chung, Julia Tan, Ashley Denmark, Thomas Gilder, John DiLeo, Katie Chang, Kevin Frank, Eli Utterback, Patrick Viviano, and Allan V. Kalueff 13. Neurophenotyping of Adult Zebrafish Using the Light/Dark Box Paradigm Adam Stewart, Caio Maximino, Thiago Marques de Brito, Anderson Manoel Herculano, Amauri Gouveia Jr., Silvio Morato, Jonathan M. Cachat, Siddharth Gaikwad, Marco F. Elegante, Peter C. Hart, and Allan V. Kalueff 14. Intraperitoneal Injection as a Method of Psychotropic Drug Delivery in Adult Zebrafish Adam Stewart, Jonathan M. Cachat, Christopher Suciu, Siddharth Gaikwad, Eli Utterback, John DiLeo, and Allan V. Kalueff 15. Assessing the Maximum Predictive Validity for Neuropharmacological Anxiety Screening Assays Using Zebrafish Amanda Linker, Adam Stewart, Siddharth Gaikwad, Jonathan M. Cachat, Marco F. Elegante, Allan V. Kalueff, and Jason E. Warnick 16. Deconstructing Adult Zebrafish Behavior with Swim Trace Visualizations Jonathan M. Cachat, Adam Stewart, Eli Utterback, Siddharth Gaikwad, Molly Hook, Kathryn Rhymes, and Allan V. Kalueff

As a model organism, zebrafish (Danio rerio) offer an opportunity to perform steadfast scientific investigations in a robust and high-throughput manner. In Zebrafish Neurobehavioral Protocols, experts in the field provide a series of detailed behavioral protocols dealing with the major current research areas in the field of behavioral neuroscience. The chapters discuss the use and interpretation of video-aided quantification of zebrafish behaviors, describe novel assays commonly used to quantify emotionality, learning, memory, and social behaviors in zebrafish, and instruct how to quantify endocrine responses and correlate them with behavior. Written in the Neuromethods series format, the chapters provide authoritative reviews of many commonly used approaches in the field today. This "how to" book will be a useful practical guide for zebrafish researchers and will complement another related book from this series, Zebrafish Models in Neurobehavioral Research. Cutting-edge and timely, Zebrafish Neurobehavioral Protocols serves as an excellent compilation epitomizing the growing potential of zebrafish in modern scientific inquiry.

-Provides detailed assays which can be performed, creatively modified, further improved, and combined in almost limitless ways -Features protocols on video-aided quantification of behavoirs, many novel assays, and the quantification of circulating cortisol levels in zebrafish -Examines ideal methods for both research and teaching laboratories